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Mycology 2017
September 25-26, 2017
conferenceseries
.com
September 25-26, 2017 Chicago, USA
2
nd
International Conference on
Mycology & Mushrooms
Volume 3, Issue 2 (Suppl)
Med Mycol Open Access
ISSN: 2471-8521
Discoveries of two new species:
Psathyrella
canalus
and
Psathyrella puntini
from low lands of Pakistan by
using molecular approach
Samina Sarwar
1
, Qudsia Firdous
1
,
and
Abdul N Khalid
2
1
Lahore College for Women University, Pakistan
2
University of the Punjab, Pakistan
Introduction:
There are a few mandatory characteristics of mushroom species that need to take into account before title them as
a novel one. In this way, morphological features including their habitat are the basic features to be noted critically and extensively.
Considering this criterion loads of new ascertains have been made throughout the world. From hilly areas of Pakistan, several
gilled and non-gilled macro fungi belonging to different orders have been reported for ages. However, this piece of study dealt with
exploration of the lowland species belonged to family Psathyrellaceae. The genus
Psathyrella
has few easily discernible characters
which leads it to be a cleaned-up genus to those that has small brown spores. The archetypal little brown mushrooms were collected
from plain areas of Pakistan falling in Punjab province.
Methods:
Initially both species were examinedmacroscopically andmicroscopically. Nonetheless, species identification has confirmed
by amplifying their conserve nuclear ribosomal internal transcribed spacer region using model fungal primers.
Results:
Final result was inferred when
P. canalus
and
P. puntini
got their separate clades showing 73 and 71 bootstrap values,
respectively, when compared among twenty-five other species. Additionally,
P. canale
had bell–shaped to convex, slightly upward
pileus margin, adnexed to adnate hymenium and soft, hollow and thick stipe were the key features that made it distinct from other
Psathyrella
species macroscopically. On the other hand,
P. puntini
was convex to umbonate and the presence of small milky–white
to lemon chiffon whitish dots all over the pileus surface was the major attribution towards its discovery. Moreover, its hymeniun was
dark–brown, adnexed to slightly narrowly adnate while stipe was milky–white to creamy white with an annulus.
Conclusions:
New discoveries are vindicated using advanced methods as here a DNA level study has proven the novelty of two new
Psathyrella
species. These findings support in evolutionary history of these particular genera, they may have applications in different
industries as well.
Samina_boletus@yahoo.comMed Mycol Open Access, 3:2, 2017
DOI: 10.21767/2471-8521-C1-003