37 / 38
Page 94
allied
academies
Ann Biol Sci, 2017
ISSN: 2348-1927
August 23-24, 2017 | Toronto, Canada
Annual Conference on
MICROBIAL PATHOGENESIS, INFECTIOUS DISEASE,
ANTIMICROBIALS AND DRUG RESISTANCE
Objectives:
The objectives of the study is to evaluate
antileishmanial activity of methanolic extract of
Aloe
otallensis
(
A. otallensis
) on the promastigote stage of
Leishmania donovani
(
L. donovani
) as compared to standard
drugs and to screen its phytochemical constituents.
Methods:
Phytochemical screening was done by using the
method mentioned by Evans and Trease on methanolic
extract of the exudates of
Aloe otallensis
leaves. The extract
was also evaluated for in vitro antileishmanial activity
against L. donavani which is found from the Parasitology Unit
of Black Lion Hospital. The result was compared to standard
drugs of sodium stibogluconate, milfostin and paramomycin.
Results:
The extract has a good antileishmanial activity
with an IC50 of 0.123 0 μg/mL on
L. donovani
(AM 563).
The experimental data showed that relatively it had better
activity than paramomycin and milfostin but less activity
than sodium stibogluconate. The data analyses were done by
GraphPad Prism version 5 software after it was read by ELISA
reader at the wave length of 650 nm. The phytochemical
screening of the exudates of
A. otallensis
showed the
presence of phenol, alkaloid and saponin.
Conclusions:
The methanol extract of the exudates of
A.otallensis has a good anti- leishmaniasis activity and this
may be attributed to phenol, alkaloid and saponin present in
the plant. But it needs further analysis for the conformation
of which constituent presents in high concentration to know
which one has the strongest effect.
e
:
zerihun.tesfaye@aau.edu.etPhytochemical screening of the exudate of
Aloe otallensis
and its effect on
Leishmania donovani
parasite
Tesfaye Zerihun
Addis Ababa University, Ethiopia
Arch Clin Microbiol, 8:5
DOI: 10.4172/1989-8436-C1-003