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Page 88
allied
academies
Ann Biol Sci, 2017
ISSN: 2348-1927
August 23-24, 2017 | Toronto, Canada
Annual Conference on
MICROBIAL PATHOGENESIS, INFECTIOUS DISEASE,
ANTIMICROBIALS AND DRUG RESISTANCE
D
ental caries is a significant public health problem which
results in destruction of the calcified tissues of tooth.
Lactobacillus
has been reported to occur in high numbers
in dental caries. The aim of this study was to screen for
the incidence of
Lactobacillus
from cases of dental caries
and to determine the antibiotic resistance profile of them.
Specimens from dental plaque were collected from 30
patients. Phenotypic and molecular methods were used for
identification of
Lactobacillus
. The antimicrobial sensitivity
test was performed to determine their resistance to 7
antibiotics. Then molecular detection of antibiotic resistance
genes was carried out using Multiplex PCR method. Out of
30 dental plaque samples,
Lactobacillus
was isolated from
14 (46/6%) of them.
mecA
gene was the most important
determinant responsible for penicillin resistance. Also
bla TEM gene had greater role in Cefazolin and Cefixime
resistance than
bla SHV. bla SHV
and
bla OXA-1
genes had
the same part in Amoxicillin-Clavulanic acid resistance. Also
tetracycline resistance caused by both
tetK
and
tetM
genes
identically. In conclusion
Lactobacillus
have important role
in the formation of dental plaque which usually shows
multidrug resistant patterns to commonly used antibiotics.
e
:
saharnouri@hahoo.comAntibiotic resistance in lactic acid bacteria isolated from human dental plaque
S Nouri Gharajalar
Tabriz University, Iran
Arch Clin Microbiol, 8:5
DOI: 10.4172/1989-8436-C1-003