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allied
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Biochem Mol biol J
ISSN: 2471-8084
Volume 3, Issue 2
Metabolomics Conference 2017
August 29-30, 2017 Prague, Czech Republic
9
th
International Conference and Exhibition on
Metabolomics and Systems Biology
Notes:
Page 33
Expression of genes involved in taxol
biosynthetic pathway in
Taxus baccata L
.
and
application of magnetic- and carbon-based
nano-adsorbents for pre-purification of taxol
Mohammad Reza Naghavi, J Nasiri, H Alizadeh
and
M R Fattahi
Moghadam
University of Tehran, Iran
T
hisworkwas undertaken toelucidate the consequences
of some environmental cues (i.e. day length,
temperature, hours of sunlight and relative humidity) on
the expression patterns of TXS, DBAT, BAPT and DBTNBT
genes contributed to the taxol biosynthetic pathway. Our
results indicated that environmental cues have synergistic
or antagonistic regulatory roles on transcription activity
and taxanes accumulation in yew, though DBAT activity
is less influenced, could be accordingly a rate-limiting
enzyme. Furthermore, a modified analytic hierarchy
process (AHP) approach based on refinement assay of
non-dominated alternatives was employed to monitor the
most reliable callus maintenance media of
T. baccata
callus cultures in terms of five criteria. Our results connoted
that L-glutamine-based feeding appears to generate more
significant results either for calli growth continuously or
taxanes production, while, for stems, both amino acid
supplies had fairly equal worth. Meanwhile, considering
decolorization efficiency, purity of taxol, recovery and
reusability of adsorbents, Fe3O4NPs@GO (50 g/L) in
dichloromethane was selected as the best medium for
pre-purification of paclitaxel. Finally, based on RSM data,
the optimum conditions to simultaneously acquire the
maximum EPPR (94.0%) and ETP (11.4%) were recorded
as adsorbent dosage of 37.7 g L
–1
, sorption temperature
of 30.7°C and agitation power of 153.1 rpm; and the
predictive results were confirmed using experimental
rechecking survey.
Biography
Mohammad Reza Naghavi is an academic member at University of Tehran,
Iran. He has published more than 140 research articles in the field of Plant
Biotechnology. He has been serving as an Editorial Board Member of four
international and national journals.
mnaghavi@ut.ac.irMohammad Reza Naghavi et al., Biochem Mol biol J, 3:2
DOI: 10.21767/2471-8084-C1-002