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Biochem Mol biol J

ISSN: 2471-8084

Volume 3, Issue 2

Metabolomics Conference 2017

August 29-30, 2017 Prague, Czech Republic

9

th

International Conference and Exhibition on

Metabolomics and Systems Biology

Notes:

Page 23

High resolution metabolomics to identify

novel biomarkers in corticosteroid resistant

asthmatic children

Youngja H Park

1

, Anne M Fitzpatrick

2

, Carl Angelo Medriano

1

and

Dean P Jones

2

1

Korea University, Korea

2

Emory University, USA

C

orticosteroid (CS) treatment is the preferred anti-

inflammatory treatment for adults and children with asthma.

However, a subset of patients fails to respond to combined

systemic and inhaled CS treatment despite very high doses and

prolonged treatment. Due to the uncertainty of the molecular

mechanism for CS-resistant asthma, this study is aimed at

discovering diagnostic biomarkers for early identification

of children resistant to CS. High resolution metabolomics

(HRM) was performed on plasma and urine samples from

CS-respondent and CS-non-respondent children to determine

putative biomarkers related to CS resistance. The metabolic

phenotypes of CS-responders and CS-non-responders were

analyzed using bioinformatics including Manhattan plot with

False Discovery Rate (FDR), Hierarchical Cluster Analysis

(HCA), Kyoto Encyclopedia Genes and Genomes (KEGG)

and Mummichog pathway analysis. The Manhattan plot with

false discovery rate determined 1894 metabolites in plasma

and 30 metabolites in urine significantly altered between

CS-responders and CS-non-responders. The important

metabolites annotated were S-adenosylmethionine (439.1395

m/z

, [M+ACN+H]

+

) and S-adenosylmethionine (378.1448

m/z

,

[M+Na]

+

) in plasma as well as

ϒ

-glutamylcysteine (236.06

m/z

, [M+S(34)+H]

+

) and Cys-Gly, (253.06

m/z

, [M-NH

3

+H]

+

),

reducedFMN(517.0794

m/z

, [M+NaCl]

+

). Thus, themetabolites

in glutathione metabolism were altered significantly regarding

CS resistance. The identified biomarkers in urine of asthmatic

children would be extremely beneficial not only for early

detection, but also in the development of therapies aimed at

preventing the irreversible airway damage and lung function

decline associated with CS resistance in severe asthma

among children.

Biography

Youngja H Park completed her MS and PhD in Pharmacology and Toxicology

under Dr. James P Kehrer at University of Texas at Austin in 1990. She

previously had worked as an Assistant Professor in the Department of Medicine

and as the Assistant Director of the Clinical Biomarkers Laboratory at Emory

University School of Medicine since 2013. At Emory University, she developed

LC-MS based metabolomics pipelines to identify novel biomarkers and the

pathways associated with diseases. She published number of metabolomics

papers in

Science and Journal of Allergy and Clinical Immunology

. Currently,

she moved back to College of Pharmacy, Korea University where she has built

metabolomics core facility after years of experience in research, evaluation

and teaching both in hospital and education institutions. Her career goal is to

identify the novel biomarkers and develop the sensors in early diagnoses of

disease.

[email protected]

Youngja H Park et al., Biochem Mol biol J, 3:2

DOI: 10.21767/2471-8084-C1-002