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Biochem Mol biol J

ISSN: 2471-8084

Volume 3, Issue 2

Metabolomics Conference 2017

August 29-30, 2017 Prague, Czech Republic

9

th

International Conference and Exhibition on

Metabolomics and Systems Biology

Notes:

Page 21

Stable isotope-assisted metabolomics of

Fusarium

head blight on wheat

Rainer Schuhmacher, Christoph Bueschl, Asja Ceranic, Maria

Doppler, Bernhard Kluger, Rudolf Krska, Alexandra Simader,

Bernhard Wolf, Barbara Steiner, Hermann Buerstmayr, Marc

Lemmens

and

Gerhard Adam

University of Natural Resources and Life Sciences, Austria

M

etabolomics studies show great potential to provide

an improved understanding of the molecular

mechanisms underlying plant diseases such as

Fusarium

head blight (FHB) on wheat. Here, we present the

successful combination of LC-HRMS based global

13

C

labeling- &

13

C tracer techniques to probe both the attack of

the mycotoxigenic fungus

Fusarium graminearum

as well

as the metabolic response of near isogenic wheat lines,

differing in a major resistance QTL against FHB. To do so,

parent and corresponding wheat NILs were cultured in the

greenhouse and treated with

F. graminearum

spores at

anthesis. In addition, custom-tailored, globally

13

C-labelled

wheat plants of the resistant and susceptible parent lines

were grown as a reference in a tailor-made labeling

chamber. To further elucidate the phenylalanine (Phe)-

and tryptophan (Trp)-derived submetabolome, wheat

ears were also treated with U-

13

C phenylalanine or U-

13

C

tryptophan (Trp) under control- and infection conditions.

LC-HRMS and subsequent data analysis clearly revealed

some 1.000 metabolites in the tested biological samples.

Among those ca. 100 and 70 were found to be derived

from the metabolic precursors Phe and Trp, respectively.

Substance with levels, which were significantly affected

by

Fusarium

have been further investigated for their

putative role in QTL mediated resistance against FHB.

The abundance of various substance classes differed

significantly between the tested wheat lines with respect

to both the timing of formation and relative amount. Our

results for hydroxycinnamic acid amides, phenolic acids,

flavonoids and lignans suggest that different molecular

mechanisms contribute to defense and resistance against

FHB. In this talk, I will present the isotope-assisted

metabolomics protocols which we have developed

and how we have investigated defense responses and

resistance mechanisms of wheat against

Fusarium

head

blight.

Biography

Rainer Schuhmacher is an Associate Professor at University of Natural

Resources and Life Sciences (BOKU), Vienna where he is heading the

working group Metabolomics and Bioactive Compounds. His research focuses

on LC-HRMS and GC-MS based metabolomics of microbes and plants

with a special focus on the interaction between these living organisms. He

received his degrees in Chemistry at University of Konstanz, Germany and

Vienna University of Technology, and in 2009 he completed his Habilitation in

Analytical Chemistry at BOKU University, Vienna. He is co-author of more than

120 SCI publications.

[email protected]

Rainer Schuhmacher et al., Biochem Mol biol J, 3:2

DOI: 10.21767/2471-8084-C1-002