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Endocrinology 2018 Journal of Clinical and Molecular Endocrinology
ISSN: 2572-5432 Page 40
August 09-10, 2018
Madrid, Spain
11th International Conference on
Endocrinology and
Diabetology
C urcumin administration reduces fertility and the serum
testosterone level in male animals. In bovine adrenal
zona fasciculata cells, curcumin treatment inhibited cortisol
production stimulated by adrenocorticotropic hormone or a
membrane-permeable cyclic adenosine monophosphate analog
by suppressing the expression levels of steroid acute regulatory
protein (StAR) and CYP11A1, which are critical for steroidogenesis.
We investigated the effect of curcumin in testosterone production.
In this study, both primary mouse Leydig cells and the MA-10
cell line were used. Enzyme immunoassay results showed that
curcumin suppressed ovine luteinizing hormone-supported
testosterone production in Leydig cells and 8-bromo-cyclic
adenosine monophosphate-supported progesterone production
in MA-10 cells. Fluo-3 AM staining results revealed that curcumin
suppressed steroidogenesis without altering long term intracellular
calcium level. Further results suggest the inhibitory effect is due
to inhibition of protein kinase A (PKA) activity. Consequently, the
expression of StAR and Cyp11A1 were suppressed by attenuating
the transcription factors Fos expression levels. In general, curcumin
inhibited transcription factor expression under cAMP stimulation,
and suppressed PKA activity and StAR and CYP11A1 expression
which obstructed steroidogenesis in Leydig cells.
Biography
Chih Hsien Chiu pursued PhD specializing in Animal Physiology, Steroidogen-
esis and Molecular Endocrinology in the Department of Animal Science and
Technology of National Taiwan University, Taiwan. Presently, he is a Profes-
sor in the same department at the same university. He continues his research
work at Academia Sinica in Taiwan.
Curcumin down regulates steroidogenesis in Leydig cells via
restriction of protein kinase A activity
Chih Hsien Chiu, Yi Chun Lina and Jyun Yuan Wanga
National Taiwan University, Taipei, Taiwan
Chih Hsien Chiu et al., J Clin Mol Endocrinol 2018, Volume 3
DOI: 10.21767/2572-5432-C2-006