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Endocrinology 2018
Journal of Clinical and Molecular Endocrinology
ISSN: 2572-5432
Page 56
August 09-10, 2018
Madrid, Spain
11
th
International Conference on
Endocrinology and
Diabetology
T
oxoplasma gondii
is an obligate intracellular parasite which
can replicate inside any nucleated cells including those of
pancreas and induces a T-cell immune response. Type-1 diabetes
(T1D) is considered as an autoimmune disease in which T-cell
mediated destruction of insulin secreting cells in the pancreas
occurs. Thus, theoretically, toxoplasmosis could play a possible
role in the development of T1D; therefore, this work was designed.
Interestingly, in the case-control study, the seropositivity of anti-
Toxoplasma
IgG was significantly higher among T1D (86.37%)
than T2D (66.67%) and the control group (60%). The experimental
study included acute and chronic Me49
T. gondii
infected mice
groups in addition to a control group. Pathological examination
revealed the presence of
T. gondii
zoites adjacent to the islets of
Langerhans of the acutely infected mice. With chronic infection,
there was a significant reduction of islets number and sizes in
association with grade-1 insulitis. The immunohistochemical
study showed significant infiltration of the islets of chronically
infected mice by CD8
+
and CD45
+
immune cells. In contrary to the
control group, the islets of the chronic group showed significantly
higher expression of the apoptotic marker caspase-3 and a
significantly lower expression of the proliferation marker Ki69.
Finally, a significant reduction of insulin expression in the islets
of chronic infection group was detected in association with a
significant increase in serum glucose concentrations; however,
the establishment of diabetes did not occur throughout this work.
Thus, this study presents an evidence for the probable role of
chronic toxoplasmosis in the development of T1D.
engy.victor77@yahoo.comToxoplasma gondii
as a possible pathogen of type-1 diabetes
mellitus: evidence from case-control and experimental studies
Engy Victor Nassief Beshay, Samar A El Refai, Mohamed A Helwa, Amany Fawzy
Atia
and
Marwa Mohammed Dawoud
Menoufia University, Egypt
J Clin Mol Endocrinol 2018, Volume 3
DOI: 10.21767/2572-5432-C2-006