Acetic Acid Treatment with Drying Treatment

Jacques Mahillon*

Department of Food and Environmental Microbiology, Earth and Life Institute, Université Catholique de Louvain, Belgium

*Corresponding Author:
Jacques Mahillon
Department of Food and Environmental Microbiology,
Earth and Life Institute, Université Catholique de Louvain,

Received date:  August 02, 2022, Manuscript No. IPRJPP-22-14814; Editor assigned date: August 05, 2022, PreQC No. IPRJPP-22-14814(PQ); Reviewed date: August 16, 2022, QC No. IPRJPP-22-14814; Revised date: August 26, 2022, Manuscript No. IPRJPP-22-14814 (R); Published date: September 02, 2022, DOI: 10.36648/ iprjpp.5.5.92

Citation:Mahillon J (2022). Acetic Acid Treatment with Drying Treatment. J Res Plant Pathol Vol.5 No.5: 092

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Although the biopesticide Bacillus thuringiensis has been widely used in pest control, large-scale production is costly due to the high costs of the medium and production method. We tried to grow Bacillus thuringiensis using inexpensive penicillin fermentation waste in this study. Under the following conditions, the waste samples from the penicillin fermentation without pretreatment, with Na2CO3, acetic acid, and drying treatments, respectively. The waste product of penicillin fermentation treated with Na2CO3 produced the highest cell counts. By cultivating B. thuringiensis 2387 in a medium containing 5% penicillin fermentation waste matter, a maximum spore count of 24.5108cfu/ml was achieved. Bacillus thuringiensis biopesticide (Bt insecticidal concentrate) had a potency of approximately 8,000 IU/mg. In the field trial, the Bt insecticidal concentrate was effective against Pieris rapae, Plutella xylostella, and Heliothis virescens to 77–90 percent.

Harmful Hemipterans in Agricultural Environment

A new option that has the potential to lessen the need for chemical pesticides is bio-pesticides, which make use of beneficial microbial agents. This study uses Cry gene content and toxicity evaluation to select and characterize Bacillus thuringiensis strains in order to make crop pest control easier. Helicoverpa armigera is the end result, a pest control polyphagous lepidopteran that is safe and effective. We selected ten soil bacteria samples from ten distinct agricultural regions in Queretaro State, Mexico; to accomplish this goal. SDS-PAGE analysis of spore-crystal mixtures confirmed the presence of 65 and 130 kDa protein bands. Maximum mortality was observed with bio pesticides whose efficacy was 4 g/L effective water. The tomato fruit borer larvae were eliminated in average by 64.88 percent. The distance between the 16S rRNA gene sequences of Bacillus thuringiensis and the PCR sequences of the bacterial strain that was compared was 98–99 percent. It is common knowledge that the toxins produced by Bacillus thuringiensis kill Lepidoptera, Diptera, and Coleoptera. However, the sap-sucking insects known as Hemiptera are not particularly vulnerable to the toxins produced by Bt. One of the most harmful hemipterans in the agricultural environment, Myzus persicae, was killed by Cry toxin from Bt strain GP919, as we show. At a concentration of 10 ng/l and a LC50 of 9.01 ng/l, the mortality bioassay demonstrates that the strain results in mortality rates greater than 80 percent; whereas Spodoptera frugiperda, a lepidopteran, was not harmed by it. Purification, solubilization, and trypsin digestion were performed on this strain's mayor protein (130 kDa), and ion-exchange chromatography was used to purify the band of 65 kDa that was used to feed the aphid. At a concentration of 10 ng/l, the bioassay reveals mortality rates greater than 85 percent and an LC50 of 6.58 ng/l. Mass spectrometry was used to find the digested fragment, and the candidate protein shared a 100% amino acid sequence with the previously identified Bt protein Cry1Cb2. We also document the signs of infection caused by the GP919 strain, which was used in Koch's postulated as well. This is the first report of a Cry1Cb2 protein that is toxic to sucking insects. This protein has the potential to become a promising, low-impact method for controlling M. persicae and possibly other pests of sucking insects.

B. Thuringiensis Strains Found in Food

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