Validation of the RP-HPLC method for analysis of captopril in pharmaceutical tablets

A simple and sensitive reversed-phase liquid chromatographic method has been developed and validated for the analysis of Captopril in bulk and its tablet dosage form. The separation was carried out on LC1(C18) coloum (250 × 4.6mm; 5μm) column at ambient temperature using methanol and water (55:45) as eluent. The flow rate was 1.0 ml/min and Captopril was quantified by absorbance at 220 nm. The retention time of Captopril was 7.20 min. The percentage recovery was within the range between 100.05 % and 100.79% for Captopril. The linear ranges were found in the range of 80μg/ml – 120μg/ml (r2 = 0.999) of Captopril. The percentage relative standard deviation for accuracy and precision was found to be less than 2%. Hence, the proposed method could be successfully employed for routine analysis of Captopril in pharmaceutical formulations according to ICH guidelines.

Author(s): B. Jebaslinhepzybai1, C. Velmurugan2 and A. Chenthilnathan1*

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