UV direct and UV Derivative spectrophotometric methods for the determination of amitriptyline hydrochloride in pure and dosage forms

A simple, sensitive and rapid UV spectrophotometric method and two UV derivative spectrphotometric methods have been developed for the determination of amitriptyline hydrochloride (ATL) in pure and its pharmaceutical formulations. The absorption spectrum of ATL standard solution (10 μg/mL) in methanol was recorded and wavelength of maximum absorbance was found to be 239.0nm. First and second derivative spectra were also recorded for the same solution. From the first derivative spectrum it is found that a valley at 252.4nm showed maximum amplitude and therefore validation of the method was carried out by measuring the amplitudes at this wavelength. Second derivative spectrum has the maximum amplitude in negative valley at 239.0nm hence the second derivative method was validated by measuring amplititudes at 239.0nm. Standard deviation and percent of relative standard deviation were calculated and found to be within the limits. The mean percent of recovery were evaluated at 50%, 100% and 150% concentration levels and found to be within the range 99.4-99.7%. The developed methods were found to be linear within the range of concentrations 5-15μg/mL, and slope, intercept and correlation coefficient were calculated and found to be satisfactory. The developed methods were found to be precise, accurate, robust and stable, therefore readily adapted for routine quality control of ATL by ordinary laboratories. The developed methods were effective for quantitative determination of ATL in bulk and pharmaceutical preparations without any interference of other constitute in tablets of different brand names.

Author(s): C. Rambabu

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