The Relationship between Single Nucleotide Polymorphism of Interleukin -10 Gene Promoter (-1082 A/G) with Recurrent Spontaneous Abortion in Iraqi Women

Background: Pro-inflammatory and anti-inflammatory cytokines and polymorphisms of their genes have been described to be involved in the pathogenesis of recurrent spontaneous abortion (RSA). Objective: To investigate the association between RSA and single nucleotide polymorphism of interleukin -10 (-1082 A/G)) in Iraqi women. Materials and Methods: The study included 350 aborted women who had history of RSA as case group, In all cases full history and complete examinations including body weight, age and body mass index were done. Furthermore, the patients were screened for various known causes of miscarriages. Results : Based on the screening test results (31.4 %) of RSA cases are unknown cause, So out of 350 only 110 case were enrolled in this study to investigate the association between RSA and promoter Polymorphisms of interleukin 10 gene (IL-10) (-1082 A/G) compared with 108 healthy controls. The frequency of the gene mutations in the patients and controls was determined using PCR-RFLP. In addition, the ELISA was conducted to investigate cytokine (IL-10) serum levels in women with RSA and healthy women. The result was shown significantly lower levels(p<0.05) in serum of IL-10 in RSA patients group than mean serum level for control group, in addition results showed no significant difference in the prevalence of IL-10 (-1082 A/G) genotype and allele frequency among women with recurrent spontaneous abortion and healthy controls in Iraqi women. Conclusion: Our findings indicate that SNP of Interleukin -10 gene promoter (-1082 A/G) is not a risk factor for RSA in Iraqi women, a suggestion which requires further studies on other cytokines.

Author(s): Hasan F. Samir and Israa A. Alwan

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