Synthesis, characterisation and spectrophotometric determination of Fe(II) complex of 2,4-dihydroxybenzaldehyde isonicotinoyl hydrazone{(E)-N’-(2,4- dihydroxybenzylidene)isonicotinohydrazide, it’s application & biological activity

analysis,Effect of diverse ion and Antimicrobial activity are studied. A simple,Sensitive and specific spectrophotometric method for the determination of Fe (II) is developed based on the colour reaction between iron (II) and 2,4-dihydroxybenzaldehyde isonicotinoyl hydrazone[2,4- DHBINH]. The optimum condition for complete colour development have been established by studying parameters like Effect of medium,Reagent concentration,Time period. Stability constant, Dissociation constant and Change in the free energy of the complex are determined. Composition of the metal and ligand has been determined by Job’s variation and mole ratio method. Application of this 2,4-DHBINH for antimicrobial activity and determination of iron in the potable water have been performed

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