Abstract

Stability indicating thin-layer chromatographic determination of fexofenadine hydrochloride as bulk drug: Application to forced degradation study

The objective of present work was to develop a validated stability indicating high performance thin layer chromatographic method (HPTLC) for estimation of fexofenadine hydrochloride. The stationary phase used was precoated silica gel aluminium plates 60 F-254 with 250 µm thickness. The mobile phase used for separation was toluene: ethyl acetate: methanol: ammonia (30%) (0.5: 7: 3: 0.6 v/v/v/v) gave a resolved peak at (Rf value of 0.27 ± 0.02). The densitometric quantification was carried out at 220 nm. Fexofenadine hydrochloride was subjected to acid and alkali hydrolysis, oxidation, dry heat treatment and photo degradation. The drug was found to degrade in acidic, alkaline, oxidative and dry heat conditions, but was found to be stable in photolytic conditions. All the peaks of degraded products were separated from the standard drug with significantly different (Rf values as 0.03± 0.02 in acid, 0.06± 0.02 and 0.84± 0.02 in base, 0.06± 0.02 , 0.62± 0.02, 0.64± 0.02 and 0.82± 0.02 in hydrogen peroxide, 0.02± 0.02, 0.68± 0.02 in dry heat conditions). The developed method can effectively separate the drug from its degradation products under accelerated degradation studies, it can be routinely employed as stability indicating method for fexofenadine hydrochloride.


Author(s): Pallavi M. Sutar, Deepali A. Bansode and Suneela S. Dhaneshwar

Abstract | PDF

Share This Article
30+ Million Readerbase
Google Scholar citation report
Citations : 374

Der Pharmacia Sinica received 374 citations as per Google Scholar report

Abstracted/Indexed in
  • Google Scholar
  • Genamics JournalSeek
  • China National Knowledge Infrastructure (CNKI)
  • Directory of Research Journal Indexing (DRJI)
  • Proquest Summons
  • International Committee of Medical Journal Editors (ICMJE)
  • Serials Union Catalogue (SUNCAT)
  • Geneva Foundation for Medical Education and Research
  • Secret Search Engine Labs

View More »

Flyer image