Spectrophometric determination of copper (II) in Biological samples by using 2–acetylpyridine 4–methyl –3-thiosemicarbazone (APMT)

2-Acetylpyridine-4-methyl-3-thiosemicarbazone (APMT) is proposed as a new sensitive reagent for spectrophotometric determination of Copper (II). APMT reacts with Copper (II) in the pH range 5 – 8 to form yellow coloured complex. The absorbance value of Cu (II) - APMT complex was measured at different intervals of time at 380 nm, to ascertain the stability of the complex. It was observed that the colour development was instantaneous and stable for more than 48 h. The system obeyed Beer's law upto 2.54 μg mL-1 of opper(II). The molar absorptivity and Sandell's sensitivity of the species is 1.475 X 104 L mol-1 cm-1 and 4 X 10-3 μg cm-2 at 380 nm. The composition of the Copper (II) complex with APMT was studied by Job's continuous variation and molar ratio method. Various biological samples and certified reference materials have been tested for the determination of Copper for evaluating the accuracy of the developed method. The results of the proposed method are in agreement with AAS method.

Author(s): K. Vasudeva Reddy, D.Nagarjuna Reddy S.Vidyasagar Babu and K. Hussain Reddy

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