Some Biochemical and Biotechnological Perspectives in Wild Chickpea and its Induced Mutants

The genus Cicer comprises 9 annual species and 31 perennial species and out of 9 annuals one species is under cultivation namely Cicer arietinum. The existing genetic variability has been harnessed in plant breeding programme which further narrowed the genetic base in cultigens. Therefore, from the variation enhancement point of view, the present study has been undertaken to assess the mutagenicity of the physical and chemical agents. The mutation breeding or mutagenesis is one of the important and useful technique and protocol to increase the mutation spectrum in the wild species which in turn could be introduced into the cultivated species to improve the qualitative and quantitative traits. SDS- PAGE electrophoresis is significance tool to assess the variation in the treated materials in form of protein profile. The seed protein profile has been studied in wild chickpea and its mutant treated with chemical and physical mutagens separately and in combination treatment and compared with the untreated parent. The analysis of the banding pattern of the control and induced mutants has been performed as per UPGMA grouping and Jaccard Similarity Coefficient. The maximum major band has been observed in the control while its mutants represent less than that of the control. The polypeptide bands shows varying size from 5.92 KDa to119.08 KDa. The genetic distance between all the 10 treatment varied from 0.333 to 0.75 as revealed by Jaccard Similarity Coefficient. The dendrogram represent one parent and 9 mutants into two major cluster in the present investigation. The induced mutants represent the deviation from the parent indicating variation when compared to the control in the present study.

Author(s): Girish. C. Kamble and H. J. Petkar

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