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Simultaneous HPLC determination of Paracetamol and Indomethacin in tablet dosage forms

A simple, specific, precise and accurate reversed phase liquid chromatographic (RP-LC) method has been proposed for the simultaneous determination of paracetamol and indomethacin in tablet dosage forms. The chromatographic separation was performed on a LiChrosorb C8, 250 mm x 4.6 mm, 5 μm column at a detector wavelength of 230 nm and a flow rate of 1.0 ml/min. The mobile phase was composed of 0.01M sodium phosphate buffer pH 7.0 and acetonitrile (35:75 v/v). The retention times of paracetamol and indomethacin were found to be 3.05 and 6.96 min, respectively. The method was validated for the parameters like specificity, linearity, precision, accuracy, limit of quantitation and limit of detection. The calibration curves were linear in the concentration range of 30.00-240.0 μg/ml for paracetamol and 2.50-20.00 μg/ml for indomethacin. The % recovery for paracetamol and indomethacin is in the range between 98.97% and 99.84% with RSD values not greater than 1.90. The presented method for the simultaneous determination of paracetamol and indomethacin in tablets is specific, rapid and simple with good sensitivity. The analytical method can be successfully adopted for quality control tests for these drugs in tablet dosage forms
Author(s): Kalin V. Ivanov, Lyudmil K. Lukanov and Gjorgji Petrov

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