Simultaneous estimation of paracetamol and lornoxicam by RP-HPLC method from combined dosage forms

A simple, rapid and precise reverse phase high performance liquid chromatographic method was developed for simultaneous estimation of Paracetamol (PL) and Lornoxicam (LO) in combined tablet dosage form. Formulation containing PL and Chromatography was performed on a 150 mm x 4.6 mm, 5-μm particle size, C8 HiQSil column using methanol: acetate buffer (60:40 v/v) mixture as a mobile phase of pH 6.5 adjusted with Glacial acetic acid. The detection of the combined dosage form was carried out at 265 nm with a flow rate of 1 ml min-1. The retention times were 1.725 & 2.725 minutes for PL and LO respectively. Linearity of the method was excellent over a concentration range 30 to 80 μg mL-1 for PL and 0.5 to 1.0 μg mL-1 for LO. The correlation coefficient was 0.9998 and 0.9994 for PL and LO respectively.The relative standard deviation values for repeatability and intermediate precision studies were less than 2 %, and % recovery was greater than 98% for both drugs. The proposed method was found to be suitable for the routine estimation of PL and LO in tablet dosage form.

Author(s): Santosh N. Mokale, Surekha S. Nirmal, Swaroop R. Lahoti, Jaiprakash N. Sangshetti

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