Simultaneous estimation of levonorgestrel and ethinyl estradiol by HPTLC in oral tablet formulation

A simple and accurate High Performance Thin Layer Chromatography (HPTLC) method has been developed for the simultaneous estimation of Levonorgestrel (LNG) and Ethinylestradiol (EED) in Tablet formulation. The chromatographic separation was achieved on aluminium plates precoated with silica gel 60GF-254 with mobile phase Dichloromethane: Methanol in ratio of9:1 %v/v measured at the wavelength of 254nm. The Rf values was found to be 0.47 and0.42 for LNG andEEDrespectively. The method was validated according to ICH guidelines and the reliability of the method was assessed by evaluation of Linearity range from1000-4000 ng/spot for LNG and 300-2100 ng/spot for EED. Recovery studiesinclude 92.43% (± 0.82%)w/w for LNG and 103.53% (±2.16%)w/w for EED.This method is simple, accurate, precise and sensitive. This method can be used for the routine analysis of these drugs in pharmaceutical formulation.

Author(s): Karnik Y. M.

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