Separation of Isoalantolactone and Alantolactone in Inula racemosa Root by RP-HPLC

A simple reversed phase high performance liquid chromatographic method has been developed, for the separation of Isoalantolactone (1) and Alantolactone (2) in the root, Inula racemosa (Pushkarmool). Resolution of 2.20 has been achieved for Alantolactone and Isoalantolactone. Linearity, with respect to the peak area of the constituents and their respective concentration was established using the regression equation, giving co-relation coefficient (r2) as 0.997 and 0.999 for 1 and 2 respectively. Repeatability (n=5) reached relative standard deviation values (R.S.D.) of 0.99% and 1.7% for the peak areas of 1 and 2, respectively.

Author(s): Meena Sharma, Alok Sharma, Rahul Singh and Chandra Kant Katiyar

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