Selective separation of trace amount of copper(II), manganese(II), cadmium(II), and nickel(II) ions with tetradentate schiff base ligand by reversed-phase high performance liquid chromatography and spectrophotometric detection.

The separation of neutral copper, manganese, cadmium, and nickel chelates of Schiff base ligand, N,N’- Bis(salicylidene)1,3-propylenediamine is reported on a column of silica. Metal chelates chelates are well resolved with good peak shape and efficiencies when the mobile phase is linear gradients of Methanol in an aqueous sodium acetate (50mmol/L, pH 5.40), using diode-array detector at 350nm.

Author(s): Nabil Bader, Milan Hutta, Heinz-Martin Kuss

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