Abstract

RP-HPLC method development for the simultaneous estimation ofatorvastatin and amlodipine besylatein bulk and pharmaceutical dosage forms

The present study was aimed to develop a high-performance liquid chromatographic (HPLC) method for atorvastatin and amlodipine besylate with high sensitivity, accuracy and precision in combined dosage form. Separation was performed on a C18 column [Agilent ODS UG 5 column, 250mm x 4.5mm], with Phosphate buffer (pH 3.4): Acetonitrile (50:50) isocratic elution with a flow rate of 1ml/min. Good sensitivity was observed with UV detection at 259nm. After method development, the interference of other active compounds and excipients, repeatability and linearity were investigated. Retention times of atorvastatin and amlodipine besylate were found to be 6.8 and 8.6 min respectively. The method was validated over the range from 100-500µg/mL for atorvastatin and 50-250 µg/mL for amlodipine besylate with correlation coefficients of 0.9997 and 0.9996 respectively. This method was shown to be accurate, robust, selective, linear, and repeatable and can be successfully employed in routine quality control for the simultaneous analysis of atorvastatin and amlodipine besylate in tablets


Author(s): Vidyadhara S., Sasidhar R. L. C., Satyaprasanna P., Ch. Aruna Kumar and ArunaKumari G.

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