RP-HPLC and UV-derivative spectrophotometry technique for the simultaneous estimation of ibuprofen and famotidine in pharmaceutical dosage form

A Reversed Phase High Performance Liquid Chromatography and UV – Derivative Spectrophotometric method has been developed for the simultaneous estimation of Famotidine and Ibuprofen in Pharmaceutical dosage forms. For RP – HPLC method, Methanol and 25 mM Heptane Sulfonate Sodium were selected as mobile phase at a flow rate of 1 ml/min using gradient elution. The detection wavelength was set at 267 nm. The responses were linear in the range of 1- 8 μg/ml for Famotidine and 40 – 180 μg/ml for Ibuprofen. The correlation coefficient for both drugs was 0.9991. The percentage recovery of Ibuprofen was 99.72 – 101.8 % and that of Famotidine was 98.2 – 99.33%. The % RSD for the precision studies was below 2%. The UV method was developed in derivative mode setting the detection wavelength for each drug at zero crossing points of the other. Famotidine was analyzed at 226 nm and Ibuprofen at 244 nm. The linearity range for Famotidine is 2- 16 μg/ml with a correlation coefficient of 0.9989 and that of Ibuprofen is 80 – 280 μg/ml with correlation coefficient of 0.9994. The percentage recovery for Ibuprofen was 98.9 – 102.2 and Famotidine was 98.25 – 99.95 % and the % RSD for the precision studies was below 2%.Both methods are reliable, accurate and precise and can be adopted for routine analysis.
Author(s): V. Rajani Sekhar, Y. Padmanabha Reddy, P. Ramalingam and D. Harihara Theja

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