Abstract

Pathologies of exocrine pancreas leading to pancreatic cancer

Acute pancreatitis (AP) is dangerous and in up to 5% of cases deadly disease with no specific cure. AP often results in the development of chronic pancreatitis (CP) and increased occurrence of pancreatic cancer (PC). The most widely recognized type of PC is pancreatic ductal adenocarcinoma and CP patients are at huge danger of creating PC. Activation of PSCs - during a pancreatic injury - induces proliferation as well as secretion of extracellular matrix components, thereby playing an important role in the fibrosis that occurs in CP and PC. The main sources of AP have been recognized as gallstone biliary malady and high liquor admission, while irregularity in calcium motioning in pancreatic acinar cells was discovered to be one of the principal occasions in this cycle. We have demonstrated already that bile acids and non-oxidative liquor metabolites instigate Ca2+overload, untimely enactment of pancreatic supportive of proteins, processing the pancreas, and its environmental factors. In this study, we explored Ca2+ signaling in the different cell types in the acinar environment of the pancreatic tissue using a pancreatic lobule preparation. We have, for the first time, recorded depolarization-evoked Ca2+ signals in pancreatic nerves and shown that whereas acinar cells receive functional cholinergic innervation, there is no evidence for functional innervations of the pancreatic stellate cells. The principal-agent bringing out Ca2+ signals in the stellate cells is bradykinin, however, in trial liquor-related intense pancreatitis, these phones become substantially less receptive to bradykinin and afterward procure an affectability to trypsin. Our new findings have implications for the understanding of the development of acute pancreatitis and we propose a scheme in which Ca2+ signals in stellate cells provide an amplification loop promoting acinar cell death. The underlying arrival of the proteases kallikrein and trypsin from passing on acinar cells can, through bradykinin age and protease-enacted receptors, induce Ca2+ signals in stellate cells which can then, possibly via nitric oxide generation, damage more acinar cells and thereby cause the additional release of proteases, generating a vicious circle. This results in AP and subsequently in CP, increasing the chances of PC.

A number of approaches have been successfully tried to alleviate alcohol-induced AP in vitro and in vivo. The inhibitor of store-operated calcium entry CM4620 (currently in the 3rd phase of human trials by CalciMedica, US) has been shown recently in our lab to reduce pathology is much lower than previously reported concentrations. Galactose is an oral supplementation has also efficiently reduced AP effects. Both approaches are currently the most promising perspectives to develop an effective AP treatment and subsequently reduce the probability of CP and PC. Our findings are beneficial for the understanding of new mechanisms that could help to combat pancreatic disorders.


Author(s): Dr. Julia Gerasimenko

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