Synthesis of TiO2 nanoparticles using optimized biomass of Planomicrobium sp., which is isolated from melted ice. Herein, the TiO2 exhibited maximum absorbance peak at 400 nm in UV Vis spectroscopy. The XRD spectrum shows the nature of the nanoparticles and the crystalline size of the nanoparticles measured using Debye-Scherer equation was used to particle size range from 8.89 nm. SEM image shows the shape of the nanoparticles. The FTIR confirmed the existence of protein as the stabilizing agent of the TiO2 particles. The nanoparticles exhibit antibacterial microbial activity against B.Subtilis, K.planticola, and A.niger.The ecofridely cost effective method was involving in this synthesis of nanoparticles.