Mesenchymal stem cells targeting endothelial cells

Cell nano-capsulation is a novel delivery system based on a self-assembly technique mediated by electrostatic interactions called Layer-by-Layer (LbL) deposition, that do not significantly increase cell/implant volume because of the nano-metric thickness of its layers. LbL depositions could coat the entire surface of individual cells, providing mechanical resistance to cells against manipulation and storage conditions prior to implantation in the patient. LbL nano-capsulation are formed over the cell by the sequential deposition of layers of polymers, which are mediated by opposite electrostatic interactions when alternating polymers of opposite charge over an, also charged, template. Using this technology, nano-capsulation of single-cells of human adipose-derived mesenchymal stem cells (ADSC) was possible and experimental factors to successfully preserve viability and functionality of cells, in order to be used in regenerative medicine applications, were assessed. Additionally, our Nano encapsulation method of mesenchymal stem cells (MSC) is useful for cell targeting by conjugating a specific antibody. Increased cell attachments over HUVEC a cell was observed by using a specific anti-CD31 conjugated on ADSC. Conjugation of a specific antibody to the nano-layer increases specific cell recognition and, possibly, tissue engraftment.

Author(s): Roberto Ebensperger

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