Investigation of the Influence of Recombinant Lactoferrin on Indicators of Lipid Metabolism in Rats with Experimental Cholestasis

Objective: The objective was to study the effect of recombinant lactoferrin milk of transgenic goats on lipid metabolism parameters-the content of acyl-glycerols, total lipids and cholesterol in some organs and tissues of rats with experimental paracetamol-induced cholestasis.

Material and Methods: Enzymatic, spectrophotometric and statistical methods are followed.

Results: The conducted studies confirm that paracetamol in large doses is able to induce cholestasis, as evidenced by an increase in the content of total lipids and acylglycerols in blood serum, liver and kidneys, cholesterol in serum and liver of rats after its single intragastric administration at a dose of 300 mg/kg. With the introduction of paracetamol and lactoferrin in different combinations, the level of cholesterol in the brain remains relatively constant and is determined within the control values.

Conclusion: It was first established that the administration of lactoferrin in a dose of 200 mg/kg for 7 days after a single injection of paracetamol at a dose of 300 mg/kg, as well as its administration at a dose of 100 mg/kg for 14 days before and at a dose of 200 mg/Kg for 7 days after paracetamol, weaken, but completely do not normalize the changes of these parameters initiated by paracetamol. It has been shown that the administration of lactoferrin (7 days) after single intragastric administration of paracetamol tends to lower the serum cholesterol level and a significant increase in its level in the liver by 50.5%. At the same time, the administration of lactoferrin (14 days) to paracetamol and (7 days) after it causes a tendency to lower the level of cholesterol in the blood serum and increase - in the liver.

Author(s): Al-Hasnawi Aman Ali

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