Wood biomass is a wellspring of crude materials for set up wood-based ventures and for the beginning biofuel segment. Productive preparing of wood fiber polymers, for example, cellulose and hemicellulose requires close observing with strategies, for example, FTIR, XPS or synthetic investigation. Such strategies are tedious and require the accessibility of particular hardware and mastery. As of late, the sugar acknowledgment areas of glycohydrolases, known as starch restricting modules, were utilized for examining the turn of events and the organic chemistry of plant cell dividers. In this investigation, we built a progression of shading coded fluorescent sugar restricting modules with specificities for four significant starch fiber polymers. This methodology named Fluorescent protein-Tagged Carbohydrate-restricting modules Method (FTCM) takes into consideration fast, high-throughput investigation of fiber surface starches marks and is in this utilized for 1-observing and anticipating the effect of different medicines on the properties of biomass mash and paper created from such prepared strands and 2-foreseeing best pretreatment procedure for different biomass buildups for biofuel creation. We accept that the effortlessness of this natural agreeable methodology could change the manner in which industry enhances biomass filaments preparing and deconstruction. Biomass is natural material that originates from plants and creatures, and it is an inexhaustible wellspring of energy.Biomass contains put away vitality from the sun. Plants retain the sun's vitality in a procedure called photosynthesis. At the point when biomass is scorched, the substance vitality in biomass is discharged as warmth. Biomass can be scorched straightforwardly or changed over to fluid biofuels or biogas that can be singed as fills. Strong biomass, for example, wood and trash, can be singed straightforwardly to create heat. Biomass can likewise be changed over into a gas called biogas or into fluid biofuels, for example, ethanol and biodiesel. These powers would then be able to be scorched for vitality. Biomass energizes gave about 5% of all out essential vitality use in the United States in 2017. Of that 5%, about 47% was from biofuels (for the most part ethanol), 44% was from wood and wood-determined biomass, and 10% was from the biomass in metropolitan waste. (Aggregate of rates is more prominent than 100% as a result of autonomous adjusting) Researchers are attempting to create approaches to utilize more biomass for fuel. There are 6 nonexclusive biomass preparing innovations. The wide scope of biomass sources accessible in nature incorporates feedstock described by various compound creations, physical status, poisonousness and vitality content. The feedstock quality speaks to an important angle impacting the choice on the most appropriate valorisation innovation to be embraced. Specifically, in spite of the vitality recuperation effectiveness ought to speak to the key driver for the decision, monetary intensity and market opportunity assume the principle job towards the business improvement of new innovations and systems. The vitality accessible in biomass might be utilized either by direct use as in burning, or by introductory updating into progressively important and helpful powers, for example, charcoal, fluid powers, maker gas or biogas. Along these lines, biomass transformation innovations can be isolated into four fundamental classifications: direct burning, thermo-compound change forms (pyrolysis, gasification), bio-synthetic procedures (anaerobic absorption, maturation) and physico-substance (the course to biodiesel). Woody biomass co-burning with coal, or direct ignition is by a long shot the most evolved part in Europe and around the world.