Extraction and quantification of Abscisic acid was carried out from ungerminated and germinated seeds of Sphenostylis stenocarpa. Abscisic acid (ABA) was extracted with 80% methanol. The hormone was purified with polyvinyl pyrrolidone (PVP) column chromatography and quantified with high performance liquid chromatography (HPLC). High performance liquid chromatography analysis identified and quantified the Abscisic acid. The result shows that ungerminated seeds contain higher quantity of ABA than germinated seeds which indicate that there was a drop in the level of ABA in germinated seeds. This study suggests that abscisic acid extracted from ungerminated and germinated seeds of S. stenocarpa could be a source of phytohormones for research and agricultural purposes.