The methanolic extract of roots of Tinospora cordifolia was screened for the wound healing activity in the form of ointment on albino mice using two types of wound models viz, excision, incision. The parameters studied include percentage of wound contraction, period of complete epithelialization and tensile strength of incision wound and histological studies of granulation tissue. The extract ointments showed considerable difference in response in the above said wound models as comparable to those of a standard drug (Povidone iodine ointment) and control (simple ointment base). Histopathological study of the granulation tissue of methanoicl extract treated animals showed few monocytes with increase in collagenation when compared to control group of animals. The results revealed that methanolic extract of roots of Tinospora cordifolia have significant (p< 0.01) wound healing activity as compared to control.