Artemisia parviflora Roxb. (syn. A. japonica; Asteraceae) is a member of Indian tarragon family and is commonly found in many areas of north western Himalayas, central Himalayas, the Nilgiri hills, Arunachal Pradesh and Uttarakhand in India. A. parviflora is a medicinal plant used in traditional system of Indian medicine for its wound healing, febrifuge, digestive, anthelmintic properties. Here, we have performed the acute oral toxicity of ethanolic extract of aerial parts of A. parviflora. In acute toxicity study total four group of mice (n=5 per group) were orally treated with doses of 0.10, 0.50, 1.0 g/kg body weight. And parameters like behavioral change, body weight change, and other abnormalities were observed. Our study showed that A. parviflora did not produce any hazardous symptoms or death in acute toxicity study showing a LD50 higher than 1 g/kg body weight. The administration of this plant material did not showed any significant changes in the body weight or any type of adverse effect on the body of animal. Hence, taken together the study proves that oral administration of ethanolic extract of aerial parts of A. parviflora produced no significant toxic effect in male and female Swiss albino mice, which could stand as an assurance for the medicinal use of this plant in folk medicine.