The plant Pluchea lanceolata (DC.) C. B. Clarke, Asteraceae, is a perennial weed of the cultivated fields of semiarid regions of India. It is found in the hotter parts of the country including Punjab, Rajasthan, Upper West Bengal and Uttar Pradesh. It is known locally as Rasna, Gandhamula, Rasya and Yuktarasa. This plant is a widely accepted, and used in the Indian system of medicine as laxative, analgesic, antipyretic, nervine tonic, aperient, for the treatment of rheumatism and allied disorders, diseases of the abdomen, dyspepsia, bronchitis, inflammation and arthritis. The present paper aims at establishing the quality control parameters for the roots of Pluchea lanceolata with various pharmacognostical and standardization techniques. The present study comprises of macroscopical and microscopical characters, physico-chemical constants, extractive values with various solvents, fluorescence analysis, its reaction after treatment with chemical reagents under visible light and UV light, preliminary phytochemical screening of root extracts, etc. as per official compendia. The results help to establish the standardisation for the drug, so as to minimise its adulteration and substitution.