Electrochemical behavior and validated determination of the azathioprine in bulk form and body fluids

The electrochemical reduction and adsorption of Azathioprine were studied in a BR buffer under different pH conditions at a Glassy Carbon Electrode (GCE). The electrochemical behavior of AZA at glassy carbon electrode was studied by using cyclic voltammetry (CV), differential pulse cathodic adsorptive stripping voltammetry (DPCAdSV), and square-wave cathodic adsorptive stripping voltammetry (SWCAdSV). The voltammogram exhibited irreversible reduction peak in acidic media, corresponding to the reduction of nitro group in the drug. The number of electrons transferred in the reduction process was calculated and the probable reduction mechanism was proposed. A systemic study of the experimental parameters that affect the square-wave stripping response was carried out and experimental conditions were optimized. The electron-transfer coefficient (α) of the electrode reaction and the diffusion coefficient (D) of the reactant species were also determined. The process was successfully applied for assay of the drug in spiked human serum and spiked urine samples.

Author(s): Gopal Lal Morya, Priyanka Sinha, Hemlata Sharma, K. K. Jhankal and D. K. Sharma

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