Determination of valifenalate fungicide residues in tomato fruit

A simple, sensitive and inexpensive method was developed using Liquid liquid extraction, together with high performance liquid chromatographic method with UV detection for determination of valifenalate residues. The evaluated parameters include the extraction procedure using different solvents and buffers (acetonitrile, n-hexane, water and 0.02 M triethylamine). The method was validated using tomato fruit samples spiked with valifenalate at different concentration levels (0.01 and 0.1 μg/mL). Average recoveries (using each concentration six replicates) ranged 88-90%, with relative standard deviations less than 2%, calibration solutions concentration in the range 0.01-5.0 μg/mL and limit of detection (LOD) and limit of quantification (LOQ) were 0.003 μg/mL and 0.01 μg/mL respectively.

Author(s): Tentu Nageswara Rao, K. Apparao, E. G. Sreenivasula Reddy, B. Gowtham Prasad and S. N. V. S. Murthy

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