A series of eleven(E)-3-[4-(Difluoromethoxy)-3-hydroxyphenyl]-1-phenylprop-2-en-1-oneswere designed and the molecular docking was carried out for this fluorochalcones with 1SA0 protein. The in silico docking studies reveal that all the compounds are bounded well with receptor via. hydrogen bond, halogen, polar, pi-pi, metal-ligand and other interactions. The chalcones were tested against bacterial strains viz. Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa.