Mucoadhesive microspheres of aceclofenac was formulated using combination of alginoeudragit RS100 system by ionic gelation technique. The prepared microspheres were evaluated for various parameters like percentage yield, particle size, flow property, surface study, ex-vivo mucoadhesive study, in vitro drug release, etc. It was found that all formulations showed improved flow behavior as compared to pure drug, it was observed that on increasing the polymer concentration of formulations the entrapment efficiency and particle size were increased. The surface morphology study by SEM indicated that microspheres were spherical with rough outer surface. There was no interaction between the drug and the polymers, as studied by FTIR study. In vitro drug release study showed that on increasing polymer concentration the drug release of all the formulations were gradually decreased and the formulations followed zero order kinetics. Ex- vivo mucoadhesion study depicts that when the polymer concentration was increased the mucoadhesion nature was also increased. Therefore, it can be concluded that aceclofenac loaded algino-eudragit RS100 microspheres can be formulated for sustained drug delivery of aceclofenac.