Dendritic cells expressing MHC Epitopes reduce Alloreactivity In-Vitro

Although immunosuppressive therapy of graft rejection and graft-versus-host disease significantly increases the prospect of successful allograft survival, it's often amid severe side effects like opportunistic infections and cancers. Therefore, the event of biological approaches to induce antigen-specific immunological tolerance to transplanted organs and tissues could be promising. In this study, we used C57Bl/6 mice dendritic cells (DCs) transfected with CBA mice MHC epitopes to suppress alloantigen-induced immune reaction in vitro. We found that C57Bl/6 DCs transfected with CBA mice MHC epitopes were capable to induce FoxP3+ Tregs and IL-10+CD4+ cells in autologous splenocyte cultures. Also, C57Bl/6 DCs transfected with CBA mice MHC epitopes suppressed the proliferation of autosplenocytes in response to CBA splenocytes but to not BALB/c splenocytes. Thus, C57Bl/6 DCs transfected with CBA mice MHC epitopes suppress immune reactions to alloantigens in antigen-specific manner.

Author(s): Valeriy Tereshchenko

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