Comparative evaluation and standardization of marketed herbal antiobesity formulation

The present study involved comparative standardization and authentification of medicinal plants and marketed herbal antiobesity formulations. Marketed herbal tablet formulation contains Vrikshamla Ghana and Triphala Ghana main ingredient. The herbal tablet formulation effective in weight loss i.e. Antiobesity effect. Formulations were standardized as per WHO, ICH guidelines and modern scientific quality control procedures. Standardization of tablet formulation was performed by physicochemical parameters, Quality control tests of tablet, IR, TLC, HPTLC. The amount of total phenols were analysed by using Folin-Ciocalteau assay. The amounts of total Flavonoid were analysed using aluminium chloride calorimetricor assay. Free radical scavenging activity was evaluated using 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) free radical. HPTLC chromatogram confirmed quantitative presence of active ingredient in herbal formulation. Total phenol was found to be 18.35mg/tablet. Total Flavonoid was found to be 1.333mg/tablet. The antioxidant activity was found to be 72%.The present standardization study reveals compliance with all the above selected parameters. The application of present study may play significant role in deciding the identity, purity, quality of the herbal tablet formulation and also for fixing standards for this Ayurvedic formulation.

Author(s): Ashwini B. Patil, Vishal V. Pandeand Prashant B. Patil

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