Comparative assessment of antibacterial activity of Tinospora cordifolia (Willd.) Miers leaves and its callus

Tinospora cordifolia (Willd.) Miers (family: Menispermaceae) is well-known for its versatile biological properties and has been extensively subjected by numerous researchers for its phyto-chemical evaluation to exploit its therapeutic potential to its maximum. Efforts have also been aimed to enhance the yield of secondary metabolites from the plant for obvious benefits. Plant tissue culture is one such technique which has been frequently employed to serve the purpose. In the present study, a comparative assessment has been carried out by subjecting leaves obtained from Tinospora cordifolia and its callus to the preliminary phytochemical as well as microbial evaluation. The MS medium supplemented with 2,4-D + 6-BA + IBA (0.5 ppm each) was found to be the best combination for the initiation and development of the callus on leaf explants. At 2 mg and 4 mg concentration of callus extract, inhibition was obtained for both the organisms. However, equal concentration of leaf extract showed inhibition of E. coli only.

Author(s): Nazeerullah Khan, Madhukar Garg, Ennus T. Tamboli, Mazibullah Khan, V. K. Sharma1, Sunil Kumar and Amit Kumar

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