The antifungal activity of petroleum ether, ethyl acetate, chloroform, methanol and aqueous extracts of the root bark of Diospyros kaki (Ebenaceae) were evaluated, against 14 human pathogenic fungi. The antifungal assay was done using agar disc diffusion method. The result showed that all the extracts except aqueous extract showed varying degree of inhibitory activity against various human pathogenic fungi. The chloroform extract showed best activity with the MIC value in the range 160-2500μg/ml. The results obtained in the present study authenticate and support the use of Diospyros kaki in folklore medicine for treatment of various skin diseases.