Isolation of secondary metabolite from Indonesian’s Marine Sponges Clathria sp., Xestospongia muta, and Endectyon delaubenfelsi against mycobacterium Desulfovibrio desulfuricans was completed on May 2018. Bioactive metabolites isolated based on bioassay-guided separation with several step of chromatography. The result of bioactivity showed that Xestospongia muta sponge has MIC50 value=0,1 μg/mL, Clathria sp. has MIC50 value=0.3 μg/mL and Endectyon delaubenfelsi has MIC50 value=0.3 μg/mL. Interpretation of the FTIR spectrum from the Xestospongia muta sponge indicate the active-metabolite has an O-H functional group at 3435.56 cm-1 with a fingerprint region of a C-O functional group at 1342.24 cm-1. The active metabolite has a C-H methyl group at 2853.39 cm-1 and C-H methylene at 2769.64 cm-1 and a C≡N imine group at 2365.28 cm-1 with fingerprint region of C-N imine functional group at 1637,27 cm-1. The Results of cytotoxic show that MIC50 value of Xestospongia muta has potential activity as the new model to the drug discovery against mycobacterium Desulfovibrio desulfuricans.