Background: It is estimated that the highest incidence of nosocomial infections prevails in the intensive care units of hospital. Following strict nursing protocols such as proper handwashing before and after patient encounter and practicing barrier nursing can help prevent spread of infection in otherwise vulnerable patients. The aim of this study is to identify the importance of handwashing and barrier nursing protocol in prevention of nosocomial infections particularly in a neuro-intensive care unit. Methods: An observational study was conducted from May 2018 to October 2018. A total of 100 patients were observed for development of any infectious complication before (n=50) and after (n=50) implementation of handwashing and barrier nursing protocol. Results: The incidence of infectious complications in patients without handwashing measures was 26% vs 10% after handwash. The most common infection was Pneumonia (10%) followed by Bloodstream and wound site infections (7% and 6%, respectively). A clear decline in rates of infections was observed following implementation of handwashing protocol. Conclusion: Among other measures to prevent complications occurring in neuro-intensive care units, the incidence and spread of nosocomial infections can be decreased by adequate hand washing and barrier nursing protocols for the staff.