Journal of Blood Research

contact@imedpub.com

Articles On Plasma Insulin

The concentration of insulin was measured by double-antibody immunoassay simultaneously within the plasma and serum of thirty-eight fasting and non-fasting individuals. The concentration found in heparin-plasma was consistently above that found in serum, and experiments suggest that the high plasma-level is an impact of heparin. Evidence from previous workers (who have measured the hormone's concentration in plasma and serum separately) would appear to support this. It is proposed that insulin should always be assayed in serum.

Patients were studied either within the fasting state or during steady-state hyperglycemia produced by endless intravenous glucose infusion. Steady-state plasma insulin concentration during these studies ranged from 10 to 264 μU/ml. Labeled insulin specific activity time curves consisted of quite one exponential, indicating that a multicompartmental system for insulin metabolism exists. A mathematical technique which is applicable to non-first order processes was wont to calculate the speed at which insulin was lost irreversibly from the plasma insulin pool. A direct, linear relationship was found between insulin irreversible loss rate and plasma insulin concentration over the range of concentrations studied. This linearity implies lack of saturability of the insulin removal mechanism. Since the plasma insulin pool was during a steady state during these studies, insulin irreversible loss rate was adequate to the speed at which newly secreted insulin was being delivered to the general circulation. Therefore, these results indicate that changes in plasma insulin concentration result from parallel changes within the rate of insulin delivery and not from changes within the other way of the rate of insulin removal. A wide range of insulin delivery rates was found among patients with similar plasma glucose concentrations, suggesting that there exists considerable variability in responsiveness to endogenous insulin among these patients.

High Impact List of Articles
Conference Proceedings

Relevant Topics in Medical Sciences

Awards Nomination
Abstracted/Indexed in
  • Secret Search Engine Labs

View More »

Open Access Journals