Journal of Organic & Inorganic Chemistry

The use of the matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF MS) technique for the development of antifungal strategies

Euroscicon Conference on Physical Chemistry and Analytical Separation Techniques
October 08-09, 2018 Amsterdam, Netherlands

Antonio Jose de Jesus Evangelista

Federal University of Ceara, Brazil

Posters & Accepted Abstracts: J Org Inorg Chem

DOI: 10.21767/2472-1123-C6-018

Abstract

In the last decades, several epidemiological studies have confirmed the increase in the frequency of invasive fungal infections (IFIs) worldwide, which are currently considered important public health problem. Clinical and experimental evidence has shown that IFIs can be caused by organized communities in the form of biofilms. These structures are characterized by intense metabolic cooperation and great resistance to physical and chemical agents and are defined as dynamic communities of microorganisms strongly adhered to a biotic/abiotic substrate and protected by a matrix rich in exopolymers, especially proteins. The extracellular matrix of the biofilm plays a crucial role in the protection of fungal cells against the immune response of the host besides being considered one of the main mechanisms of resistance to antifungals. Understanding the mechanisms involved in the synthesis of the biofilm matrix is the key point for the development of antifungal strategies. There are several techniques for identifying the components of the biofilm matrix, one of them is the matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), which allows analyzing the biofilm matrix constituents. Thus, this communication aims to emphasize the use of the MALDI-TOF MS technique as a tool for the development of antifungal strategies.

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