Journal of Orthodontics & Endodontics

Nanofibrous asymmetric collagen/curcumin membrane containing aspirin loaded plga nanoparticles for guided bone regeneration

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Amirhosein Bani Shahabadi

Faculty Tabriz University of Medical Sciences, Iran

ScientificTracks Abstracts: J Orthod Endod

Abstract

Bone defects are considered among the common devastating problems worldwide. Although bone grafting has been applied in the clinic to solve this issue, it has been reported that the rapid migration of fibroblasts to the defect or fracture site can shape a fibrous capsule which prohibits the efficient growth of blood vessels and osteoblasts into the graft tissue and finally result in bone nonunion. New developed guided bone regeneration (GBR) methods which effectively halts bone destroying and improves new bone generation hold promise in this context. Creation and maintenance of a secluded space via a barrier membrane is a fundamental rule in GBR which inhibits invasion of the rapidly growing fibrous capsule and other soft tissues and thus facilitated the inhabitance of host bone-originated osteoblasts in defect niche. High biocompatibility, satisfactory degradation and mechanical specifications in order to produce proper barrier functionalities, space maintenance and clinical handling should be the essential properties of used membranes. Enhancing these properties and the ability to complete new bone regeneration via membrane materials is an important research field in bone regeneration. Nanotechnology is the use of a substance on an atomic, molecular and supramolecular scale and nano- medicine deals with the medical implementation of nanotechnology. Nanotechnology-based production of asymmetric membranes is considered to be a perfect bone healing which provides a top layer that acts as an inhibitory barrier against pathogen invasion and physical damages and also an inner layer which allows bone resorption and maintains the niche that is crucial for bone regeneration. The aim of the current study was to design an asymmetric membrane including aspirin-loaded PLGA nano- particles and curcumin nanofibers using nanotechnology methods for guided bone regeneration purposes. Material and Methods (Patient report or search method or method of evaluation): The membrane was prepared using electrospinning technique and then was physic chemically characterized by the conventional methods. The release profile of aspirin from the prepared membrane was also measured by ultraviolet spectrophotometry. Also, the antibacterial activities of the membrane was evaluated. We also assessed the in vitro effects of the prepared membrane on the biocompatibility and osteogenic differentiation of dental pulp stem cells (DPSCs), and evaluated in vivo bone regeneration using the prepared membrane in the defects created in both sides of the dog’s jaw by histology.The institutional ethics committee of the Tabriz University of Medical Sciences agreed with both theses and all experimentations were done based on the related guidelines and rules. The code of ethics for preparation and physicochemical assessments was IR.TBZMED. REC.1397.317. For cellular and in vivo investigations the code of ethics was IR.TBZMED.VCR.REC.1399.164. Findings: The results from the characterization specified that the membrane was successfully prepared with monodispersed nanosized fibers, uniform network shaped morphology, negative surface charge and sustained release platform for aspirin. The membrane also showed antimicrobial effects against all tested bacteria. The presence of curcumin and aspirin in the asymmetric membrane enhanced osteogenic potential at both transcriptional and translational levels. The results of the animal test showed that the test area was completely filled with new bone after just 28 days, while the commercial membrane area remained empty. There was also a soft tissue layer above the new bone area in the test side. Conclusion: We suggested that the prepared membrane in this work could be used as a GBR membrane to keep soft tissue from occupying bone defects in GBR surgeries. Besides, the surgeries can be benefited from antibacterial activities and bone healing effects of this novel GBR membrane while, simultaneously, promoting bone regeneration. Key words: Guided bone regeneration, Membrane, Nanotechnology.

Biography

Dr.Amirhosein Bani Shahabadi D.D.S, O.M.F.S has completed his D.D.S. at the age of 28 years from Qazvin University of medical science ,Iran and post graduated studies of oral and maxillofacial surgery from Tabriz University School of Medical science ,Iran. He is the CEO of Amlak Teb Avizhe, an inovative virtual surgery company and also he is an inventor that has a patent in Iran . He has published more than 9 papers in reputed journals.