Journal of Organic & Inorganic Chemistry

N-(2-Hydroxyethyl) morpholine HCL salt content by hydrophilic interaction liquid chromatography (HILIC) with charged aerosol detection (CAD)

EuroSciCon Conference on Chemistry 2018
February 19-20, 2018 Paris, France

D Wang and P glynn

Alkermes, USA Alkermes, Ireland

ScientificTracks Abstracts: J Org Inorg Chem

DOI: 10.21767/2472-1123-C1-002

Abstract

The utility of charged aerosol detection (CAD) for quantitation has received considerable attention in the pharmaceutical industry. The operating principle of drying and applying a charge to particles in the eluent stream which are directly measured at an electrometer provides a repeatable response when analyzing compounds which lack a UV chromophore. Due to the molecular chemistry of the morpholine alcohol HCL salt, it was found that neither traditional HPLC-UV nor GC-FID analysis was capable of detecting residual amounts present in the drug substance. Further development studies examining different combinations of mobile phases, organic additives, columns (C8, C18, and HILIC) with a CAD detector were performed. It was determined that HCL salt format of a morpholine compound could be successfully analyzed by an HPLC �?¢�?�?�?�?CAD method with a HILIC column. The method proved capable of controlling residual morpholine alcohol residual in a drug substance.

Biography

D Wang has completed his PhD from Florida International University. He is a Senior Scientist at Alkermes, a global biopharmaceutical company. He has published more than 12 papers and presentations in reputed journals and scientific conferences.
Email:david.wang@alkermes.com