Isolation of male sterile and maintainer lines from North Indian onion (Allium cepa L.) populations with the aid of PCR based molecular marker

Joint Event on 3rd World Congress on NATURAL PRODUCTS CHEMISTRY AND RESEARCH & 12th WORLD PHARMA CONGRESS
October 16-18, 2017 Budapest, Hungary

Geetika Malik, Ajmer Singh Dhatt and Ajaz Ahmed Malik

ICAR-Central Institute of Temperate Horticulture, India Punjab Agricultural University, India Sher-e-Kashmir University of Agricultural Sciences and Technology, India

Posters & Accepted Abstracts: Am J Ethnomed

DOI: 10.21767/2348-9502-C1-003

Abstract

Marker Assisted Selection (MAS) using mitochondrial DNA based marker cytochrome b (cob) was integrated with phenotypic evaluation to isolate male sterile and maintainer lines from open pollinated onion varieties adapted to North Indian agroclimatic region. Cytotype (N/S) determination by cob marker followed by morphological and microscopic study of pollen discovered male sterile plants (Smsms) at frequencies of 0.015 in Punjab Naroya, 0.020 in Punjab Selection, and 0.006 in Punjab White. The progeny scoring of test-crosses between male sterile and N-cytoplasmic plants isolated the maintainers (Nmsms) at frequencies of 0.133 in Punjab Naroya, 0.231 in Punjab Selection and 0.182 in Punjab White. As a novel approach, Trait Recovery Programme was demonstrated to reduce the population size required to recover a male sterile plant by 91.08% in Punjab Naroya, 92.99% in Punjab Selection and 97.66% in Punjab White. For recovering a maintainer, 10% reduction in Punjab Naroya and 9.10% in Punjab Selection was calculated. However, no reduction was observed in Punjab White. This analysis also validated that in a randomly mating onion population, frequency of recessive ms allele squared is equal to the frequency of male sterile plants among S-cytotype and frequency of maintainers among N-cytotype (fms2 = fSmsms/fS = fNmsms/fN).

Biography

Geetika Malik is currently working as an Scientist and Assistant Professor in the division of vegetable science at Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, India. She is expertise in Natural Products and Horticulture.

E-mail: [email protected]