Insights in Neurosurgery

Iron homeostasis and hepcidin quantification in Alzheimers disease patients

4^th International Conference on Neurodegenerative Disorders and Stroke
July 05-06, 2017 Frankfurt, Germany

Manolov V, Hadjidekova S, Petrova J, Vasilev V, Petrova M, Kuntchev T, Jelev Y, Jeliazkov P, Tzatchev K and Traykov L

Medical University Sofia, Bulgaria
Aleksandrovska University Hospital, Bulgaria

ScientificTracks Abstracts: Neurosurg

DOI: 10.21767/2471-9633-C1-002

Abstract

Background: Alzheimerâ�?�?s disease (AD) is characterized by deposition of amyloid plaques of amyloid-�?² chelating peptide with transition metal ions (Cu2+, Zn2+ и Fe3+). The binding of Cu2+ and Fe3+ leads to toxic chemical reactions; a change in the oxidation of two metals, that leads to H2O2 production in the presence of transition metals and finally gives toxic free OHâ�?¢ radicals. Methods: 41 Alzheimerâ�?�?s disease patients were included in this study. They were evaluated for serum iron, copper, selenium, zinc and hepcidin levels. Superoxide dismutase (SOD) and glutathione peroxidase (GPX) were measured as oxidative stress markers. Hepcidin, SOD and GPX were measured by ELISA methods. Serum Fe, Cu, Se and Zn were quantified by AAS. The results form AD patients were compared to age and gender matched healthy controls. We used Pearsonâ�?�?s correlation and Studentâ�?�?s paired t-test for statistical analysis of established results. Results: We found statistically significant elevated serum iron, copper and zinc results in AD patients (42.9 �?¼mol/l, 39.7 �?¼mol/l, and 41.1 �?¼mol/l) compared to control group (21.5 �?¼mol/l, 18.7 �?¼mol/l, and 15.9 �?¼mol/l); P<0.01. Plasma selenium levels were decreased in AD patients (121.7 nmol/L) compared to healthy controls (652.4 nmol/L); P<0.005. Hepcidin concentrations were increased in AD cases (61.1 �?¼g/l) compared to controls (20.7 �?¼g/l); P<0.001. SOD and GPX levels were decreased in Alzheimerâ�?�?s disease (8.9 �?¼g/ml, and 11.4 pg/mL) compared to normal values in healthy controls (21.7 �?¼g/ml; and 39.5 pg/mL); P<0.001. Conclusions: The expected contribution from our study is practical introduction of quantification of serum hepcidin as a potential marker for early diagnosis of impaired iron homeostasis, leading trace element in the pathogenesis of neurodegenerative diseases.

Biography

Manolov V has completed his PhD from Medical University in Sofia, Bulgaria. He is working as Assist. Prof. at Department of Clinical laboratory and clinical immunology at the same University. He has interests in iron metabolism, gynecology, neurology, endocrinology and pediatrics. He has published more than 25 papers in reputed journals and participated in more than 60 National and International meetings in different medicine fields.

Email: victhedoc3@mail.bg