Koloko Brice L, Kenmogne Hubert, Ngaha Njila M, Bogning Calvin, Wankeu Modeste, Bushra Ijaz, Dongmo Alain and Massoma Dieudonné
University of Douala, Cameroon University of the Punjab, Pakistan
Posters & Accepted Abstracts: Am J Ethnomed
Objective: This study was aimed to investigate the effect of ethanolic extract of Rauvolfia vomitoria stem bark on sexual behavior and male reproductive function in normal rats.
Methods: 25 healthy sexually experienced Albino male rats of 3-4 months, weighing between 190 and 220 g were randomly divided into five groups (A–E) of five rats each and orally treated once daily for 22 days. Group A (control) received 5 mL/ kg body weight of distilled water, groups B, C and D received 50, 100 and 200 mg/kg BW of Rauvolfia vomitoria extract respectively, while group E received 5 mg/kg BW of sildenafil citrate (standard). Sexual behavior parameters including mount frequency (MF), intromission frequency (IF), ejaculation frequency (IF), mount latency (ML), intromission latency (IL), ejaculation latency (EL) and post-ejaculatory interval (PEI) were recorded in male rats one hour after treatment by mating with a receptive female (1:1) at day 0, 1, 8, 15 and 22. At the end of treatment, body and organ weights, histological analysis and sperm parameters were also evaluated.
Results: The treatment with Rauvolfia vomitoria extract improves sexual behavior through significant reduction of ML, IL and PEI (p<0.01) and significant increase of EL, IF and EF (p<0.01) as compared to control. The extract also significantly increased daily sperm production rate (DSP) and epididymal sperm counts (p<0.001) as well as sperm transit (p<0.05) compared to control. Testis histology showed that the extract increased the concentration of all germ cell types, Leydig cells and Sertoli cells as compared to control.
Conclusion: Present findings provide experimental evidence that the ethanolic extract of Rauvolfia vomitoria enhances male sexual activity and production function in rats.
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