Effects of ethanolic extract of Rauvolfia vomitoria (Apocynaceae) stem bark on sexual behavior and reproductive function in normal male rats

Koloko Brice L; Kenmogne Hubert; Ngaha Njila M; Bogning Calvin; Wankeu Modeste; Bushra Ijaz; Dongmo Alain; Massoma Dieudonné

doi:10.21767/2348-9502-C1-003

Effects of ethanolic extract of Rauvolfia vomitoria (Apocynaceae) stem bark on sexual behavior and reproductive function in normal male rats

Joint Event on 3^rd World Congress on NATURAL PRODUCTS CHEMISTRY AND RESEARCH & 12^th WORLD PHARMA CONGRESS
October 16-18, 2017 Budapest, Hungary

Koloko Brice L, Kenmogne Hubert, Ngaha Njila M, Bogning Calvin, Wankeu Modeste, Bushra Ijaz, Dongmo Alain and Massoma Dieudonné

University of Douala, Cameroon University of the Punjab, Pakistan

Posters & Accepted Abstracts: Am J Ethnomed

DOI: 10.21767/2348-9502-C1-003

Abstract

Objective: This study was aimed to investigate the effect of ethanolic extract of Rauvolfia vomitoria stem bark on sexual behavior and male reproductive function in normal rats.

Methods: 25 healthy sexually experienced Albino male rats of 3-4 months, weighing between 190 and 220 g were randomly divided into five groups (A–E) of five rats each and orally treated once daily for 22 days. Group A (control) received 5 mL/ kg body weight of distilled water, groups B, C and D received 50, 100 and 200 mg/kg BW of Rauvolfia vomitoria extract respectively, while group E received 5 mg/kg BW of sildenafil citrate (standard). Sexual behavior parameters including mount frequency (MF), intromission frequency (IF), ejaculation frequency (IF), mount latency (ML), intromission latency (IL), ejaculation latency (EL) and post-ejaculatory interval (PEI) were recorded in male rats one hour after treatment by mating with a receptive female (1:1) at day 0, 1, 8, 15 and 22. At the end of treatment, body and organ weights, histological analysis and sperm parameters were also evaluated.

Results: The treatment with Rauvolfia vomitoria extract improves sexual behavior through significant reduction of ML, IL and PEI (p<0.01) and significant increase of EL, IF and EF (p<0.01) as compared to control. The extract also significantly increased daily sperm production rate (DSP) and epididymal sperm counts (p<0.001) as well as sperm transit (p<0.05) compared to control. Testis histology showed that the extract increased the concentration of all germ cell types, Leydig cells and Sertoli cells as compared to control.

Conclusion: Present findings provide experimental evidence that the ethanolic extract of Rauvolfia vomitoria enhances male sexual activity and production function in rats.