Cucurbitacin D inhibited pro-inflammatory cytokine production and proliferation in keratinocytes

Heung Mook Shin; In Jun Yang

doi:10.21767/2348-9502-C1-006

Cucurbitacin D inhibited pro-inflammatory cytokine production and proliferation in keratinocytes

6^th Edition of International Conference on Pharmacognosy and Medicinal Plants
April 16-17, 2018 Amsterdam, Netherlands

Heung Mook Shin and In Jun Yang

Dongguk University, South Korea

Posters & Accepted Abstracts: Am J Ethnomed

DOI: 10.21767/2348-9502-C1-006

Abstract

Psoriasis is a chronic, relapsing inflammatory skin disease, characterized by erythematous plaques covered by silvery scales. While the pathogenesis of psoriasis is still unclear, most findings indicated that intensifying T helper 1(Th1) and Th17 are required for the development of psoriasis. Cucurbitacin D is chemically classified as steroid with anticancer activity on endometrial and ovarian cancer cells. First, we evaluated the effects of cucurbitacin D on inflammatory mediator and chemokine production in IL-1α, IL-17A, IL-22, oncostatin M, and TNF-α (named M5 combination)-stimulated 3D reconstituted human epidermis. Cucurbitacin D significantly inhibited the production of IL-1β, IL-20 and GM-CSF. Second, we examined whether cucurbitacin D influences the expression of proliferation marker in IL-22 stimulated HaCaT keratinocytes. Cucurbitacin D significantly suppressed the expression of K16, K17 and Ki67 in IL-22 stimulated HaCaT cells at a concentration of 30μM. Cucurbitacin D also suppressed M5 induced phosphorylation of ERK 1/2 in HaCaT cells. These results suggest that cucurbitacin D may be useful as an anti-inflammatory agent, especially for psoriasis.